Hohenbuehelia lageniformis sp. nov., Phonemany, Raspé, Thongkl, & K.D Hyde. Figure 4–5

Face of fungi number: FoF 10709, Mycobank: XXX

Etymology: Lageniformis (Latin) is referred to its lageniform shape of cheilocystidia.

Holotype: MT44, MFUL xxx.

Diagnosis: This species distinguished from other with fleshy, gelatinous zone obviously visible pale brown to light brown and mucilaginous basidiomata, white velutinous hair at the base, remaining part yellowish white dense woolly when young becoming spares when old, and visually whiteish hair cover margin zone. Its spores subglobose. Pileipellis consisting cutis and tufts of a trichoderm. by the diagnostic of ITS, LSU, and TEF1 sequence data.

Pileus 25–30 × 15–25 mm, young stage sapathuliform, rounded flabelliform to sub rounded flabelliform when mature, laterally attached or sometimes sessia, pale orange to orange white (5A2–5A3), greyish orange (5B4) becoming darker with edge, margins discolorous from pileus, rolled then becoming involved when old, entire or sometimes wavy when old, surface moist, shiny with white hair (1A1) velutinous at the base, then yellowish white dense woolly when young becoming spares when old, and visually whitish hair cover margin zone observed under lens. Lamellae 1–2 mm wide, decurrent, some radiating from point of attachment, close to sub-distant when young and becoming moderated crowded when old, 4–5 lamellulae length, white to pale orange (5A1–5A2) then becoming dark with age.  Stipe absent or sometimes pseudostipe 0.5–1 mm long present when young and disappeared when mature stage. Context consisting of two-layer, fleshy, white leathery layer 1 mm wide, and gelatinous layer 1–2 mm wide, soft, sticky, brown to light brown and mucilaginous.  Order mild. Test tasteless. Spore prints white.

Basidiospores (6.9–)7.3–8.8–10(–10.5) × (5.5–)5.9–7–8.1(–8.3) µm subglobose, to ellipsoid, smooth, inamyloid, thin-walled. Basidia (19.3–)20.8–31.7–53.1(–58.2) × (5.4–)5.5–9.4–13.9(–14.6) µm, clavate, with 4 sterigmata, 4–8 µm long, 2–4 spores, hyaline, smooth, thin-walled. Cheilocystidia (17.6–)17.6–21.9–26.5(–26.9) × (3.2–)3.2–5–8.7 µm, lageniform with an inflated base with a thin, rostrate apex, hyaline, thin-walled. Pleurocystidia (30.7–)31.2–50.3–70.3(–70.5) µm, metuloidal, setiform, less broad to narrowly fusiform, with encrusted with crystals, brownish or yellowish in water, hyaline present crystals in 5% KOH with thick-walled. Context consisting of gelatinized zone with compound hyphae 1.6–4.5 µm wide, thick as revived in 5 % KOH. Hymenophoral trama subregular. Pileipellis consisting of an interrupted cutis with hyphae 1.96–5.27 µm wide, and tufts of hairs near the base present a trichoderm, with long cylindrical terminal elements 41–122 µm in length. Trichodermial elements (Pileoleptocystidia) absent. Clamp connections present in all observed tissues.

Habitat: Solitary, gregarious to imbricate growing on decayed wood in tropical wet forest, rain forest in Thailand.

Specimens examined: THAILAND, Chiang Mai province, Mae on district, Tabol Huay keo, Pox village, 29th June 2019, Monthien Phonemany (MT44, MFLU xxx); Chiang Rai province, Mae Yao district, Huay Mae Suk waterfall, 10th July 2019, Monthien Phonemany (MT59, MFLU xxx, MT58, MT66, MFLU xxx); Pa Ngea village, Pa Dead district, Chiang Rai, 7th September 2019, Monthien Phonemany (MT164, MFLU xxx).

Notes: There are many studies published by the authors referring to Hohenbuehelia lageniformis being different from all hither to known species (Stevenson 1964, Fazio & Albertó 2001, Liu & Bau 2009, Silva-Filho & Cortez 2017, Consiglio & Setti 2018, Consiglioet al. 2018, Holec & Zehnalek 2020). H. odorata was originally described from India by Bijeesh et al. (2019) based on morphology and molecular data (ITS sequence). Figure 1 shows Hohenbuehelia lageniformis and H. odorata are closely related with similar morphology; i.e yellowish-white, medium and leathery, spathulate to flabelliform basidiomata. But H. odorata differ by having smaller basidiospores (5.2–7.6 × 4.8–6.4 µm) and gelatinous matrix of subpellis present cylindrical to flexuous of pileoleptocystidia. Hohenbuehelia lageniformis is visually different. It has yellowish-white velutinous hair at the base and woolly whitish hair cover margin zone and larger basidiospores (6.9–)7.3–8.8–10(–10.5) × (5.5–)5.9–7–8.1(–8.3) µm), pileoleptocystidia absent. Furthermore, there was a difference of 0.88% in the ITS sequence of Hohenbuehelia lageniformis and H. odorata.

Fig. 4: Basidiomata and a gregarious of Hohenbuehelia lageniformis on substrate. a–d= MT44 (MFLUXXX); d= MT59 (MFLUXXX); e–f= MT 164 (MFLUXXX); (scale bar= 2 cm).

Fig. 5 Micro-morphological of Hohenbuehelia lageniformis. a = Basidiospores, b = Basidia, c = Pleurometuloids, d = Cheilocystidia, e = Cutis of pilieipellis, f = a trichoderm of Pilieipellis (scale bar a–d = 10 µm, e = 20 µm, f = 10 µm).