Helgardiomyces anguioides (Nirenberg) Crous, comb. nov.

MycoBank number: MB 838073; Index Fungorum number: IF 838073; Facesoffungi number: FoF14536;

Basionym: Pseudocercosporella anguioides Nirenberg, Z. PflKrankh. PflSchutz 88: 246. 1981.

Synonyms: Ramulispora herpotrichoides var. anguioides (Nirenberg) U. Braun, Nova Hedwigia 56(3–4): 433. 1993. Ramulispora anguioides (Nirenberg) Crous, S. Afr. J. Bot. 61: 47. 1995. Helgardia anguioides (Nirenberg) Crous & W. Gams, Eur. J. Pl. Path. 109: 846. 2003. Oculimacula anguioides (Nirenberg) Crous, IMA Fungus 5: 103. 2014.

Typus: Germany, Göttingen, culm base of Triticum aestivum, 1979, H. Nirenberg (holotype B); culture ex-holotype BBA 64003 = CBS 496.80.

Notes: Colonies differ from those of Oculimacula in that they are fast growing, and dull pinkish on PDA, velvety, with entire margin. Conidia are long and flexuous, up to 280 µm in length, and pluriseptate (Nirenberg 1981). In contrast to Oculimacula, H. anguioides is weakly aggressive to wheat (Bateman 1988). The species formed a fully or well-supported lineage in most analyses of the loci for which sequences were available (ITS, act, rpb2, tef1; Figs 2, S1, S3, S5, S6; multigene phylogenies Figs 2,S7–S9), with the exception of LSU where it was not well resolved (Figs 1, S2).

Fig. S1. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the ITS sequence alignment of sequences generated in this study and reference sequences from NCBI GenBank. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. S2. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the LSU sequence alignment of sequences generated in this study and reference sequences from NCBI GenBank. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. S3. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the act sequence alignment of sequences generated in this study and reference sequences from NCBI GenBank. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. S5. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the rpb2 sequence alignment of sequences generated in this study and reference sequences from NCBI GenBank. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. S6. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the tef1 sequence alignment of sequences generated in this study and reference sequences from NCBI GenBank. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. S7. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the full set 6-gene (ITS, LSU, act, tef1, rpb1, rpb2) sequence alignment. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. S9. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the full set 2-gene (rpb1, rpb2) sequence alignment. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and culture collection numbers are indicated. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072).

Fig. 1. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the LSU sequence alignment. Bayesian posterior probabilities (PP) > 0.89 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Families, orders and classes are indicated with coloured blocks to the right of the tree. GenBank accession and/or culture collection numbers are indicated behind the species names. The tree was rooted to Candida broadrunensis (GenBank KY106372.1) and the taxonomic novelties described in this study are indicated in bold face.

Fig. 1. (Continued)

Fig. 2. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the reduced set 6-gene (ITS, LSU, act, tef1, rpb1, rpb2) sequence alignment. Bayesian posterior probabilities (PP) > 0.85 are shown at the nodes and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Species and genera are indicated with coloured blocks to the right of the tree. Culture collection numbers are followed by the host and country of origin where known. The tree was rooted to Ascocoryne sarcoides (culture NRRL 50072) and the taxonomic novelties described in this study and cultures with a type status are indicated in bold face.