Haudseptoria typhae   Crous   &   R.K.   Schumach.,   sp.   nov.

MycoBank number: MB 839288; Index Fungorum number: IF 839288; Facesoffungi number: FoF 11954; Fig. 29.

Etymology: Name refers to the host genus Typha from which it was isolated.

Conidiomata erumpent to semi-superficial, dark brown, globose, 100–150 µm diam with central ostiole, 10–15 µm diam; wall in upper region of brown textura angularis, in lower region of pale brown cells, concolourous with host tissue. Conidiophores reduced to conidiogenous cells, ampulliform to subcylindrical, smooth, 5–12 × 2–3 µm, proliferating sympodially at apex. Conidia solitary, hyaline, smooth, guttulate, subcylindrical, apex subobtuse, base truncate, filiform, straight to slightly curved, (1–)3-septate, septa thin-walled, (16–)20–23(–27) × 2(–2.5) µm in vivo; 35–90 × 3 µm, 3–7-septate in vitro.

Culture characteristics: Colonies erumpent, spreading, with sparse aerial mycelium and smooth, even margin, reaching 12 mm diam after 2 wk at 25 °C. On MEA surface buff, reverse cinnamon; on PDA surface cinnamon, reverse honey with patches of cinnamon; on OA surface buff.

Typus: Germany, near Berlin, moist meadow, on dead leaf sheath of Typha sp. (Typhaceae), 17 Apr. 2019, R.K. Schumacher, HPC 2978 = RKS 249 (holotype CBS H-24407, culture ex-type CPC 38203 = CBS 146790).

Notes: Haudseptoria represents a new septoria-like genus (Verkley et al. 2013) related to Pseudopassalora gouriqua (hyphomycete on Protea suzannae, South Africa; Crous et al. 2011), and Phellinocrescentia guianensis (coelomycete with aseptate conidia, on polypores in French Guiana; Crous et al. 2014).

Based on a megablast search of NCBI’s GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Didymosphaeria futilis (strain CMW 22186, GenBank EU552123.1; Identities = 487/521 (93 %), 13 gaps (2 %)), Italiofungus phillyreae (strain CPC 35566, GenBank MT223804.1; Identities = 446/499 (89 %), 16 gaps (3 %)), and Funbolia dimorpha (strain CPC 14170, GenBank JF951136.1; Identities = 405/467 (87 %), 18 gaps (3 %)). Closest hits using the LSU sequence are Pseudopassalora gouriqua (strain CBS 101954, GenBank NG_067272.1; Identities = 785/808 (97 %), no gaps), Italiofungus phillyreae (strain CPC 35566, GenBank MT223899.1; Identities = 784/808 (97 %), no gaps), and Eriomyces heveae (strain MFLUCC 17-2232, GenBank MH109524.1; Identities = 779/810 (96 %), two gaps (0 %)) – also see Fig. 1. No significant hits were obtained when the tub2 sequence was used in blastn and megablast searches.

Authors: P.W. Crous, J.Z. Groenewald & R.K. Schumacher

Fig. 1, parts 1–5. Consensus phylogram (50 % majority rule) resulting from a Bayesian analysis of the Dothideomycetes LSU nucleotide alignment. Bayesian posterior probabilities (PP) > 0.79 are shown at the nodes and the scale bar represents the expected changes per site. Thickened branches represent PP = 1. The branch leading to Superstratomycetales was halved to facilitate layout. Families and orders are indicated with coloured blocks to the right of the tree. GenBank accession (superscript) and / or culture collection / voucher numbers are indicated for all species. The tree was rooted to Diaporthe perjuncta (voucher BPI 748437, GenBank NG_059064.1) and the species treated in this study for which LSU sequence data were available are indicated in bold face.

Fig. 1. (Continued).

Fig. 1. (Continued).

Fig. 1. (Continued).

Fig. 1. (Continued).

Fig. 29. Haudseptoria typhae (CPC 38203). A. Conidioma on host tissue. B. Conidiomatal ostiole. C. Conidiogenous cells giving rise to conidia. D.

Conidia. Scale bars: A = 150 µm, all others = 10 µm.