Crinipellis trichialis (Lév.) Pat. ex Antonín, Ryoo & H.D. Shin, Mycotaxon 108: 432 (2009).
Index Fungorum number: IF 543142; Mycobank number: MB 543142; Facesoffungi number: FoF 12910; Fig. 1
Pileus 5–18 mm in diameter, hemispherical to convexcylindrical when young, becoming convex to plano-convex or applanate with age, disc with a tuft of scales forming a small papilla, with concentric zones of erect fibrils or scales around the disc, margin fibrillose; papilla and central zone dark brown (6F6–7F8), middle zone brown (6F6–7F8), margin brownish yellow (5C5–5C8). Context<1 mm thick, white. Lamellae adnexed to free, close to crowded with 2–4 series of lamellulae, white to pale yellowish white (3A1–3A2). Stipe 5–25 × 0.5–1.5 mm, central, cylindrical, equal with an enlarged base, tough, pliant, fibrillose to hairy overall, insititious, brown to dark brown. Rhizomorphs absent.
Basidiospores 6–11 × 5–8 μm, Q = 1.0–2.0 (n = 50), ellipsoid, smooth, hyaline, inamyloid, thin-walled. Basidia 18–35(–40) × 6–8 μm, clavate, 4-spored. Pleurocystidia 30–60×6–10 μm, clavate, hyaline, inamyloid, thick-walled. Cheilocystidia 20–32×5–10 μm, clavate, simple or a majority with 1–4 apical appendages, hyaline, inamyloid, thin-walled. Pileipellis a cutis, 5–6 μm in diam, hyaline, inamyloid, with spiral encrustations, giving rise to terminal hairs with basal clamp connections. Hairs 50–460×2–5 μm diam, cylindrical, apex rounded to acute, with secondary septations, thick-walled, dextrinoid, yellowish-brown to greenish brown in KOH. Stipitipellis composed of repent cortical hyphae and terminal hairs; cortical hyphae 1–3 μm in diam, cylindrical, dextrinoid, light yellow brown to green in KOH; medullary hyphae 3–5 μm in diam, cylindrical, strongly dextrinoid, hyaline; terminal hairs 100–325 ×7.5–10 μm, cylindrical, apices acute, with secondary septations, dextrinoid, yellowish brown to greenish brown in KOH. Clamp connections present.
Habitat and distribution – Solitary to gregarious, on decomposing wood and sticks (mostly found in bamboo). Known from Brazil, Indonesia, Malaysia, Thailand and Venezuela (Kerekes and Desjardin 2009; This study).
Material examined – Thailand, Lamphun Province, Sri Bua Ban subdistrict, Chiang Mai University Haripunchai Campus, 18°31′59″N 99°7′46″E, elevation 401 m, solitary to gregarious on decaying wood, 16 August 2020, J. Kumla and N. Suwannarach, SDBR-CMUNK0900 (new record for Thailand).
GenBank number – ITS=OP503450 (SDBR-CMUNK0900).
Notes – Phylogenetic analysis indicated that Crinipellis trichialis separated from sister taxon C. scabella and C. rhizomaticola (Fig. 2). Crinipellis scabella differs from C. trichialis by negative KOH reaction (Singer 1943). The narrower size of basidiospores in C. rhizomaticola (8.5–10×4–5.25 μm, Antonín et al. 2009) clearly distinguishes it from C. trichialis (6–11×5–8 μm).
Figure 1 – Crinipellis trichialis (SDBR-CMUNK0900). a Basidiomes. b Basidiospores. c Basidia. d Cheilocystidia. e. Pileipellis hairs Scale bars: a=10 mm, b‒e=10 μm
Figure 2 – Phylogenetic tree derived from maximum likelihood analysis of ITS gene of 20 sequences and the aligned dataset was comprised of 782 characters including gap. The average standard deviation of the split frequencies of the BI analysis was 0.00637. A best scoring RAxML tree was established with a final ML optimization likelihood value of -3314.6448. The matrix had 347 distinct alignment patterns with 18.53% undetermined characters or gaps. Estimated base frequencies were found to be: A=0.2579, C=0.1949, G=0.2212, T=0.3258; substitution rates AC=1.0835, AG=3.5775, AT=1.5611, CG=0.3860, CT=4.5691, GT=1.0000. Marasmius iras KLU M 73 and Marasmius ochropoides KLU M 89 were used as outgroup. Numbers above branches are the bootstrap statistics percentages (left) and Bayesian posterior probabilities (right). Branches with bootstrap values≥70% and PP≥0.90 are shown at each branch. The bar represents 0.1 substitutions per nucleotide position. Type strains are in bold. The newly generated sequences are indicated in blue