Clematidis italica Tibpromma, Camporesi&K.D. Hyde, sp. nov.
Index Fungorum number: IF 551868, Facesoffungi number: FoF 01814, Fig. 1
Etymology: Name reflects the country, where this species was collected
Holotype: MFLU 14–0669
Saprobic on Clematis vitalba L. in terrestrial habitats. Sexual morph Ascomata 170 – 182 μm high × 137 – 168 μm diam. (x̄ = 174 × 149 μm, n = 5), superficial, solitary or scattered on the host surface, globose to subglobose, with flattened base, ostiole in the center, not easy to removed, black, without papilla. Peridium 14 – 20 μm wide, composed of several layers of brown to dark brown, flattened pseudoparenchymatous cells, arranged in a textura angularis. Hamathecium of 1.3 – 1.7 μm wide, long cylindrical, cellular, anastomosed, septate, pseudoparaphyses. Asci 79 – 114 × 13 – 18μm (x̄ = 93 × 15 μm, n = 15), 8-spored, bitunicate, cylindrical to cylindric-clavate, rounded at the apex, short pedicellate or sessile. Ascospores 21 – 30 × 5 – 8 μm (x̄ = 26 × 6 μm, n = 20), overlapping 2 – 3 – seriate, hyaline, fusiform, straight or slightly curved, 1 – septate in center, slightly constricted at the median septa, swollen with large guttules in each cell, lacking a mucilaginous sheath, smooth – walled. Asexual morph Undetermined.
Culture characteristics: on MEA reaching 2 cm diam. after 2 weeks at 16 °C, later with dense mycelium, with irregular colony, edge undulate, surface smooth with raised elevation, white-gray; hyphae septate branched, grey, thin – walled.
Material examined: ITALY, Corniolino, Santa Sofia, Forlì-Cesena Province, on dead stem of Clematis vitalba (Ranunculaceae), 2 March 2013, Erio Camporesi, IT1086 (MFLU 14–0669, holotype); ex-type living culture, MFLUCC 15–0084); Ibid. (HKAS92499 bis, paratypes).
Fig. 1 Clematidis italica (holotype) a Appearance of ascomata on host substrate. b Section of ascoma c Section of peridium d Pseudoparaphyses e – h Ascus with minute pedicel I – k Ascospores l Germinated spore. Scale bars: a = 200 μm, b = 50 μm, c = 10 μm, d = 2 μm, e – h = 20 μm, i – l = 5 μm.