Chlorophyllum globosum (Mossebo) Vellinga, Mycotaxon 83: 416 (2002)

Index Fungorum number: IF 374394; MycoBank number: MB 374394; Facesoffungi number: FoF 07069; Figs. 1, 2

Pileus up to 150 mm diam., first subglobose, expanding to convex and plano-concave when mature, with straight margin, when young glabrous, brownish grey to light brown (7D3–8), soon surface broken and leaving concolorous glabrous at center, with concolorous patches spreading towards margin, on white to yellowish-white (4A2) fibrillose background, margin white, sulcate and exceeding lamellae when mature. Lamellae free, white to orange-white (5A2) at first, becoming grayish green (29B4) with age, very crowded, ventricose, 10–16 mm wide, with eroded lamellae-edge. Stipe 140–180×10–20 mm, tapering to apex, with wide bulb at base, 25–30 mm wide; smooth and white background, becoming orange grey to greyish orange (6B2- 3) with age. Annulus cuff-like, moveable, white in upper part, with brownish grey to light brown (7D3–8) squamules at underside. Context thick and white in pileus, white in stipe, turning pastel red (9A4) in both pileus and stipe when cut. Smell and oduor not observed. Spore print grayish-green (29D5–6). Basidiospores [25,1,1] 8.5–12.5×6.5–8.5 µm, avl×avw=10–11.5×7.5–8 µm, Q=1–1.5, avQ=1, broadly ellipsoid to ellipsoid-amygdaliform in side-view, broadly ellipsoid or amygdaliform in frontal view, apex truncate and with germ pore, thick-walled, hyaline, dextrinoid, congophilous. Basidia 18–350 × 8–16 µm, clavate, hyaline, slightly thick-walled, 4-spored. Cheilocystidia abundant, 35–62 × 8–15 µm, clavate to narrowly clavate, slightly thick-walled, hyaline. Pleurocystidia absent. Pileus covering a hymenoderm made up of several layers of cylindrical
hyphae, terminal elements cylindrical to narrowly clavate with rounded or attenuate apex 50–950×4–13 µm, slightly thick-walled, hyaline or with pale yellow intracellular pigment. Clamp connections absent.

Material examined – LAOS, Vientiane Capital, Xaythany,  Houay Yang Preserve Forest, 16 June 2017, P. Sysouphanthong, PS2017-7 (HNL503445, new record).

GenBank numbers – ITS=MN317315.

Notes – We observed the specimens of Chlorophyllum globosum from young to mature stages. Chlorophyllum globosum is a tropical species originally described from Cameroon (Mossebo et al. 2000). It is distributed in other tropical countries such as China, India, South Africa and Thailand (Ge et al. 2018). It closely resembles C. molybdites in morphology, but the phylogenetic analysis showed that they are two different species (Vellinga 2003). This study suggests the first record of Chlorophyllum globosum for Laos (Figs. 1, 2 and 3).

Figure 1 – Chlorophyllum globosum (HNL503445, new record). a–c Basidiomata

Figure 2 – Microcharacters of Chlorophyllum globosum (HNL503445). a Basidiospores. b basidia. c Cheilocystidia. d Element cells at pileus covering. Scale bars: a–c=10 µm, d=20 µm

Figure 3 – Maximum likelihood phylogenetic tree of Chlorophyllum based on nrITS sequences. The maximum likelihood (ML) analysis was performed in RAxML 7.2.6 (Stamatakis et al. 2008) and maximum parsimony (MP) analysis was performed the program PAUP* 4.0 b10 (Swofford 2004). Bootstrap values of ML/MP equal to or greater than 70% are given above branches. GenBank accession number is indicated after species name. Agaricus bisporus (FJ223228) is an outgroup. The three shows six different sections. Newly sequences generated from Laos are in blue