Cancellidium Tubaki

MycoBank number: MB 7483; Index Fungorum number: IF 7483; Faceoffungi number: FoF;

Saprobic on wood and twigs in freshwater habitats. Asexual morph: Colonies on natural substrates effuse, black, glistening, scattered. Mycelium mostly immersed, composed of branched, septate, smooth, thin‑walled, hyaline to pale brown hyphae. Conidiophores semi‑macronematous to macronematous, mononematous, pale brown to yellowish brown, smooth, thick‑walled, septate, cylindrical, sometimes developed from assimilative hypha. Conidiogenous cells monoblastic, integrated, terminal, pale brown to yellowish brown. Conidia acrogenous, holoblastic, solitary, dry, olivaceous to greyish green, shiny, flattened, obovate or ellipsoidal, fan‑shaped, mostly with truncated head, composed of many parallel, septate columns arranged in lines radiating from conidial base, internally contain chains of subhyaline, small moniliform cells. Sexual morph: Undetermined.

Type species: Cancellidium applanatum Tubaki

Notes: Cancellidium was established by Tubaki (1975) and included two species (Index Fungorum 2020). The type species, C. applanatum was collected from wood blocks in Japan. Putative collections of this species were later found in Australia, Brazil, Brunei, China, India, Malaysia, Peru and Thailand and commonly in freshwater (Sivichai et al. 2002; Fryar et al. 2004; Pinnoi et al. 2006; Zhao et al. 2012; Zelski et al. 2014, Table 3). In the phylogenetic analysis with five gene regions (ITS, LSU, SSU, TEF1‑α and RPB2) (Fig. 4), the three new taxa of Cancellidium nested within the genus with strong support (100MLBS/100MPBS/0.99PP, Fig. 4). Cancellidium cinereum (MFLUCC 18‑0424) clustered as a sister taxon to C. applanatum (CBS 137653, CBS 137655 and CBS 377.76) and Cancellidium sp. (MFLUCC 14‑0290) with good support (96 MLBS/91MPBS/0.97PP, Fig. 4). Cancellidium atrobrunneum (MFLUCC 20‑0100) grouped with strain of GUFCC 18002 which was identified as C. applanatum (Pratibha et al. 2014). In this study, we recognized this strain as C. atrobrunneum based on both phylogeny and morphology. Cancellidium griseonigrum (MFLUCC 17‑2117) formed a distinct clade within Cancellidium (Fig. 4). Based on both phylogenetic analysis (Figs. 1, 4) and morphological characters, we introduce three additional species to Cancellidium.

Fig. 1 Phylogenetic tree based on RAxML analyses of combined LSU, SSU, TEF1‑α and RPB2 sequence data. Maximum likelihood bootstrap ≥ 75% (MLBS) and Bayesian posterior probabilities ≥ 0.95 (PP) are indicated at the nodes. The new species are in red and ex‑ type strains are in bold. The tree is rooted with Chlorociboria aeruginosa (AFTOL‑ID 151) and Dermea acerina (AFTOL‑ID 941)

Fig. 1 (continued)

Fig. 4 Phylogenetic tree based on RAxML analyses of combined ITS, LSU, SSU, TEF1‑α and RPB2 sequence data. Maximum likelihood bootstrap ≥ 70%, Maximum parsimony ≥ 70% and Bayesian poste‑ rior probabilities ≥ 0.95 (BYPP) are indicated at the nodes