Canalisporium caribense (Hol.-Jech. & Mercado) Nawawi & Kuthub., Mycotaxon 34(2): 479 (1989)

≡ Berkleasmium caribense Hol.-Jech. & Mercado 1984

Index Fungorum number: IF 125432; MycoBank number: MB 125432; Facesoffungi number: FoF 05486; Fig. 1

Saprobic on submerged decaying wood in the river. Sexual morph Undetermined. Asexual morph Colonies on natural substrate sporodochial, scattered, punctiform, granular, black, shining. Mycelium immersed, composed of branched, septate, pale brown to brown. Conidiophores micronematous or semi-macronematous, mononematous, fasciculate, simple or sometimes branched, septate, hyaline to very pale brown. Conidiogenous cells monoblastic, integrated, terminal, determinate, hyaline or pale brown. Conidia 21–42 × 18–26 μm (x̅= 32.5 × 24 μm, n = 30), acrogenous, muriform, solitary, broadly ellipsoidal to obovoid, fusiform to obclavate, flattened, brown to dark brown, one longitudinal column septum, thick and dark at the septum, 9–13-transverse septate, constricted at the septa, smooth-walled.

Culture characteristics – Conidia germinating on water agar and germ tubes produced from basal conidium within 24 h at room temperature. Colonies growing on PDA, circular, umbonate, slightly fimbriate to entire edge, olivaceous grey in the middle, with dark brown at the margin, dark in reverse. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown.

Material examined – THAILAND, Phayao Province, Pong District, Yom River, on decaying submerged wood in the river shore, 18 December 2019, S.K.U. Chandrasiri and S. Boonmee, YR1 (MFLU 21-0072, new record), living culture, MFLUCC 21-0095.

GenBank numbers – ITS=MZ538501, LSU=MZ538535, TEF1-α: MZ567080.

Notes – A new collection of Canalisporium caribense (MFLUCC 21-0095) was collected from a freshwater river in northern Thailand (Fig. 1). Our collection shares identical morphology to the type species C. caribense (HolubováJechová and Mercado 1984; Nawawi and Kuthubutheen 1989). Further, phylogenetic analysis based on a combined LSU, ITS, SSU, TEF1-α and RPB2 sequences place our isolate MFLUCC 21-0095with strains of C. caribense (SS03683 and SS03839; Sri-indrasutdhi et al. 2010) with 99% MLBS, 1.00 BYPP support (Fig. 2). Therefore, a new additional record of C. caribense is reported from northern Thailand.

Figure 1 – Canalisporiumcaribense (MFLU 21-0072, new record). a–c Sporodochia on wood. d, e Squash mount of sporodochia. f Conidiogenous cells with conidia. g–j Conidia. k Germinated conidium. l, m Culture on PDA from surface and reverse. n Mycelium on PDA. Scale bars: a–c=20 μm, d–n=5 μm

Figure 2 – Maximum likelihood phylogenetic tree based on a combined LSU, ITS, SSU, TEF1-α and RPB2 sequence data of genera in Savoryellaceae. Conioscypha aquatica MFLUCC 18-1333 and C. submersa MFLU 18-1639 are selected as the outgroup taxa. Bootstrap support values for maximum likelihood (MLBS, left) equal to or greater than 70% is given above the nodes. Bayesian posterior probabilities (BYPP, right) equal to or greater than 0.95 are given above the nodes. Ex-type strains are in bold and newly generated sequences are in blue