Camposporium ramosum Whitton, McKenzie & K.D. Hyde, Fungal Divers. 11: 177–187 (2002)
Index Fungorum number: IF 489895; Mycobank number: MB 496895; Facesoffungi number: FoF 16039 (Fig. 1)
Saprobic on submerged decaying petiole on Livistona chinensis. Sexual morph: Not observed. Asexual morph: Hyphomycetous. Colonies on natural substrate, effuse, brown, velvety. Mycelium immersed and superficial, septate, fasciculate, unbranched. Conidiophores 55–90× 5–8 μm (x̅=71.6 × 6.9 μm, n=10) macronematous, mononematous, unbranched, erect, irregularly cylindrical, dematiaceous towards the base, fading to pale brown towards the apex, septate, thick-walled. Conidiogenous cells holoblastic, polyblastic, integrated into the apical region of the conidiophores, lighter the color towards the top, from brown to hyaline, denticulate; cylindrical denticles act as separating cells, 1–3 denticles per conidiophore. Conidia 40–90 μm long × 8–15 μm (at the widest point) (x̅=51.9 × 9.0 μm, n=40), solitary, easy to dry, cylindrical, elongate, brown or pale brown, concolorous or with 2 cells at each end paler in pigmentation, slightly thickened walls and septa, 7–9-septate, apex rounded, basal cell truncate, often with a persistent portion of the denticle attached, the apical cell gives rise to a single, simple or branched appendage; appendage hyaline, aseptate, smooth, tapering from the base to the apex, appendage is located near the base and is usually divided into 2 (sometimes 1) terminal branches, individual branches 20–40 μm long.
Culture characteristics – colonies on PDA reach 7 cm diam. at 25℃ after five days. Upper view wrinkled, filamentous, entire margin, flat, cloudy, fluffy for aerial hyphae, become grey-black with time, dense for aerial hyphae, the reverse becomes black.
Material examined – China, Yunnan Province, Qujing City, QuJing Normal University, decaying petiole on Livistona chinensis, 5 August 2022, Y.R. Xiong, XG075 (MHZU 23-0132)—living culture ZHKUCC 24-0053=ZHKUCC 24-0054.
GenBank numbers – ZHKUCC 24–0053: ITS=PP620704; LSU =PP620707; SSU =PP620709; tef1-α=PP668964. ZHKUCC 24–0054: ITS=PP620705; LSU =PP620708;
SSU=PP620710; tef1-α=PP668965.
Notes – In the present study, our two isolates (ZHKUCC 24–0053 and ZHKUCC 24-0054) obtained from Livistona chinensis, clustered together with C. ramosum (CBS 132483, KUNCC 10792, KUNCC 10793) with 99% ML values and 1.00 BYPP bootstrap support. The nucleotide difference between the strain of (ZHKUCC 24-0053) and C. ramosum (CBS 132483) revealed that 0.01% in LSU (1/850 bases), and 0.05% in ITS (3/522 bases) excluding gaps. The conidia size of ZHKUCC 24-0053 are not as large as C. ramosum (CBS 132483), and only 7–9-septate (40–90×8–15 μm vs. 80–112×6.4–9.6 μm; 7–9-septate vs. 8–15-septate). However, the color distribution and morphology of the apical appendages of the C. ramosum (CBS 132483) and ZHKUCC 24-0053 are similar. Based on morphology and molecular data analysis, we identified our collections are C. ramosum and this collection represents a novel host record of the fungus on L. chinensis from China (Figs. 1, 2).
Figure 1 – Camposporium ramosum (MHZU 23-0132 new host record). a Substrate; b–c colonies on wood; d–f conidiophores; h–j conidia; k germinating conidium. Scale bars: b, c 50 mm; d–f 10 μm; h–j 20 μm; (k) 50 μm
Figure 2 – Phylogram generated from maximum likelihood analysis based on combined ITS, LSU, SSU and tef1-α sequence data representing the species of Melanommataceae. Related sequences are taken from Calabon et al. (2021). Twenty-four strains are included in the combined sequence analysis, which comprised 3302 characters with gaps (ITS=519 bp, LSU=829 bp, SSU=990 bp, tef1-α=964 bp). The tree topology of the maximum likelihood analysis is similar to the Bayesian analysis. The best RAxML tree with a final likelihood value of − 14,767.515593 is presented. The evolutionary model GTRGAMMA is applied for all the genes. The matrix had 876 distinct alignment patterns with 28.01% of undetermined characters or gaps. Bootstrap support values for ML equal or greater than 70% and Bayesian posterior probabilities greater than 0.90 are given near nodes respectively. The tree is rooted with Cyclothyriella rubronotata (CBS 419.85; TR9) in Cyclothyriellaceae. The ex-type strains are indicated in bold. The newly generated sequence is indicated in blue