Calycina papaeana Lestari & K.D. Hyde, sp. nov.
Index Fungorum number: IF 900437; MycoBank number: MB 900437; Facesoffungi number: FoF13908; Fig. 1
Etymology – Epithet represents Pa Pae sub-district where the specimen was collected.
Saprobic on decaying root of an unidentified plant. Sexual morph: Apothecia 165–487 × 145–450 µm (x = 143.4 × 229 µm, n = 10), dark yellow in dry condition, arising singly or in small cluster, stipitate. Receptacle cupulate, olivaceous dark brown to black. Stipe 1–2×0.5–1 mm in dry condition, concolorous with receptacle, rugose. Disc yellow, shallow cupulate, slighly concave. Ectal excipulum 23–50 µm (x = 31.8 µm, n = 10), composed of thin-walled, hyaline to pale brown cells of textura porrecta. Medullary excipulum 23–57 µm ( x = 39.2 µm, n = 10) in lower flanks, composed of thin-walled, hyaline cells of textura oblita. Hymenium 62–75 µm ( x = 70.1 µm, n = 10), hyaline. Paraphyses 1.2–2.3 µm in diam. (x = 1.7 µm, n = 10), filiform with occasional subclavate swells at the apex, sometimes branched at the base, guttulate. Asci 38–58 × 3–6 µm ( x = 50.2 × 4.6 µm, n = 10), 8-spored, unitunicate, cylindrical, rounded, amyloid, with long substipitate base, arising from croziers. Ascospores 4–9 × 2–5 µm (x = 5.7 × 3.2 µm, n = 15), ellipsoid, uniseriate or partially biseriate, hyaline, biguttulate, rounded at both ends. Asexual morph: Undetermined.
Material examined – Thailand, Chiang Mai Province, Pa Pae sub-district, Mushroom Research Center, 8 September 2020, Anis S. Lestari, CM 11 (MFLU 23-0015, holotype)
GenBank numbers – ITS: OQ079546, LSU: OQ079547
Notes – The ITS and LSU sequences of our collection (MFLU 23-0015) show Calycina species as the closest matches with NCBI. Our collection forms a distinct lineage in the combined ITS and LSU sequence analyses with ML/BI=91%/0.99 statistical support (Fig. 2). The nucleotide differences in ITS and LSU sequences of our collection and phylogenetically close Calycina species are listed in Table 3. Our collection morphologically fits with Calycina by having
gelatinized ectal excipulum, filiform paraphyses, cylindrical asci with apical rings and ellipsoidal ascospores (Baral and Marson 2005; Baral and Rämä 2015; Suija and Motiejūnaitė 2017; Lestari and Chethana 2022).
Figure 1 – Calycina papaeana (MFLU 23-0015, holotype). a Examined material. b Apothecia on the substrate. c Vertical cross-section through the apothecium. d Close up of hymenium at the margin e Ectal excipulum cells. f Paraphyses. g–i Asci (i. mounted in Congo red). j Crozier at the base of a young ascus (arrowed). k Ascal tip (J+without KOH treatment). l–n Ascospores. Scale bar: b=380 µm, c=200 µm, d=60 µm, e=25 µm, f–n=10 µm
Figure 2 – The best scoring RAxML tree with a final likelihood value of − 8091.026 based on the combined ITS and LSU sequence data of taxa in Pezizellaceae. The tree is rooted with members of Cenangiaceae (Chlorencoelia torta JAC 14068, KUSF 52256 and ICMP 21732). The tree topology of RAxML is identical with Bayesian analysis. Sixty strains were included in the data set which comprised 1322 characters (466 characters for ITS and 854 characters for LSU including the gap regions). The matrix had 901 distinct alignment patterns with 68.15% undetermined characters and gaps. Estimated base frequencies were as follows: A=0.2424, C=0.1082, G=0.3617, T=0.9371; substitution rates AC=2.2394, AG=3.3421, AT=2.2394, CG=1.0, CT=8.6577, GT=1.0; gamma distribution shape parameter α=0.688. The new species is indicated in blue bold. Bootstrap value (ML) is greater than 70% and Bayesian posterior probabilities (BI) greater than 0.90 are given at the nodes