Aspergillus subramanianii Visagie, Frisvad & Samson, Stud. Mycol. 78: 55 (2014)

Index Fungorum number: IF 809203; Mycobank number: MB 809203; Facesoffungi number: FoF 16043 Fig. 1

Associated with dead spider Myrmaplata sp. Sexual morph: undetermined. Asexual morph: Hyphomycetes, Conidiophores biseriate, Stipes hyaline to brown, rough walled, 373–1310 × 6.5–10 μm (x̄= 840 × 8, n). Vesicles globose, 30–34 μm wide; Metulae 8–12×3.5–5.5 μm, covering 100% of head, minor proportion only 75%; Phialides ampulliform, 7.2–10.6 × 2.2–3.5 μm. Conidia globose, smooth, 2.4–3 × 2.4–3 μm (2.4±0.1 × 2.4±0.1, n=30).

Culture characteristics – Colonies growing after 7 days at 25 °C on MEA: 45–48 mm in diameter; Colony surface floccose to somewhat velutinous; mycelial areas white; sporulation light yellow (4A5); soluble pigment absent; exudate absent; reverse light brown to brown (5D7–6D7).

Material examined – India, Kasaragod, Karadka (12°32′28″N 75°08′11″E), on dead spider Myrmaplata sp., 23 July 2022, Rajeshkumar K. C. and Sruthi O. P., AMH 10632, living culture NFCCI 5694.

GenBank numbers – NFCCI 5694: ITS: OR807407, BenA: OR824932, CaM: OR824933.

Notes – In the phylogenetic analysis of a combination of ITS-CaM-BenA shows our isolates clustered with A. subramanianii with 100% ML-BS (IQ TREE), 100% R-ML, BYPP=1.00 support. Aspergillus subramanianii was introduced by Visagie et al. (2014) from shelled Brazil nuts, in Canada. Our strain colonised on dead insects was
collected from Karadka village of Kasargod, India. Macro and micromorphological characters fit well with the description of A. subramanianii in the biseriate conidiophores with yellow conidia. Hence, we determine our isolate as a new collection of A. subramanianii from a new host and a new geographical record to India.

Figure 1 – Aspergillus subramanianii (NFCCI 10632, new record) a-b Colonies on natural substrate (on insect). c-d Conidiophores. e Conidia. f Rough walled conidiophores. g Colony on MEA (obverse). h Colony on MEA (reverse). Scale bars: a-b=100 μm, c=20 μm, d-f=10 μm