Allocanariomyces tritici Mehrabi, Asgari & Zare, sp. nov. (Fig. 2)

MycoBank number: MB 835854; Index Fungorum number: IF 835854; Facesoffungi number: FoF;

Etymology: Named after the host genus from which this fungus was isolated.

Ascomata maturing on PCA within 20 days, at first hyaline, then becoming black, non-translucent, superficial, globose to subglobose, solitary, non-ostiolate, glabrous, 100–130 μm diam. Rhizoids poorly developed, brown, septate, up to 60 μm long and 1–2.5 μm diam. Ascomatal wall pale brown, with cells arranged in a textura epidermoidea when observed in surface view. Asci spherical, ovate or pyriform, eight- spored, thin-walled, evanescent, spore-bearing part 20–36 × 18–25 μm (av. = 28 × 22 μm, n = 20), with stalks 5–10 μm long. Ascospores one-celled, gray-brown, fusiform, with attenuated ends, densely granulated, 13–22.8 × 9–16 μm (av. = 18 × 11.8 μm, n = 30), with a distinct, subapical germ pore. Conidia arising terminally or laterally from hyaline to brown aerial hyphae or short branches of hyphae up to 1 μm long, blastic, globose to pyriform, hyaline to brown, smooth, solitary, 3–9× 3–4.5 μm (av. = 4.9 × 3.6 μm, n = 20).

Culture characteristics: Mycelium composed of branched, septate, smooth, hyaline hyphae, partly becoming brown in advancing regions, 1.5–3.7 μm wide. Colonies on PCA attaining 38 mm diam. in 7 days at 25 °C, circular, flat, at first hyaline, becoming buff (45); reverse of the same color. Colonies on PDA attaining 15 mm diam. in 7 days at 25 °C, circular to slightly irregular, slightly raised, wrinkled at the center, glabrous, dense, often deeply immersed into the agar, buff (45); reverse of the same color. Colonies on OA attaining 9 mm diam. in 7 days at 25 °C, circular, flat, usually fasciculate at the center and glabrous towards the periphery, grayish- white; reverse buff (45).

Holotype: Iran, East Azerbaijan province, Hashtrud, 37° 30′ 17.6″ N, 46° 59′ 42.11″ E, seed endophyte of Triticum boeoticum, Sept. 6, 2018, M. Mehrabi (IRAN 17711F); ex- type culture, IRAN 3450C.

Other strain examined: Iran, East Azerbaijan province, Hashtrud, 37° 30′ 17.6″ N, 46° 59′ 42.11″ E, seed endophyte of Triticum boeoticum, Sept. 6, 2018, M. Mehrabi (IRAN 4014C).

Fig. 2 Allocanariomyces tritici (IRAN 3450C). a Colony on PDA after 1 month at 25 °C. b Part of the colony on PCA, showing mature perithecia. c, d Perithecia. e Outer surface of the perithecial wall. f, g Asci. h Ascospores. i Hyaline and brown hyphae. j–l Conidia. Scale bars: b = 500 μm; c, d = 100 μm; e–h, j–l = 10 μm; i = 20 μm