Acanthohelicospora pinicola Boonmee & K.D. Hyde, sp. nov.,Index Fungorum number: IF550573,

Etymology: Epithet named for the host, Pinus.

Holotype: MFLU10–0049

Saprobicon dead wood of Pinus.Sexual state: Ascomata 116–148 µm high × 108–138µm diam. ( = 129.5 × 122 µm), superficial,solitary, scattered, globose to subglobose, black, ostiolate, surrounded by 39–72 × 5–7.5 µm, black setae which taper to an acute apex. Peridium 12–18.5 µm wide, composed of several layers, outer layer of compressed and black-walledcells, inner layer comprising brown-walled cells of textura angularis. Hamatheciumcomprising numerous, ca. 2 µm wide, filiform, septate, branched, hyalinepseudoparaphyses. Asci 131.5–183 × 14–18 µm ( = 150 × 16 µm), 8-spored, bitunicate, cylindrical, pedicellate, with thick and rounded apex, lacking an ocular chamber. Ascospores 41.5–57 × 3–4 µm ( = 50.5 × 3 µm), overlapping fasciculate, long fusiform-cylindrical, straight to slightly curved, 7–8(-9)-septate, not constricted at any septum, hyaline, smooth-walled. Asexual state:hyphomycetous, helicosporous.Conidiophores mononematous, erect, 3–5 µm long × 2–4 µm wide, pale brown to brown, smooth-walled. Conidiogenous cells holoblastic, terminal or intercalary, dentate, smooth, with a thickened and truncate conidiogenous loci. Conidiahelicosporous, (53-)65–92(-99) µmlong × 16–20(-25) µm diam., filaments 2 µm wide, when coiled, curved and coiled in 1–2 dimension, elongate, tapering to narrowly rounded ends, multiseptate, not constricted at the septum, hyaline, guttulate, smooth-walled.

Cultural characteristics: Ascospores germinating on MEA within 12 h and germ tubes produced at both ends. Colonies slow growing on MEA, reaching less than 5 mm in 7 days at 28C, slightly convex, undulating to raised, dentate, with slightly radial striations and lobate edges, brown. Asexual state produced in culture.Mycelium superficial, composed of branched, septate, smooth, hyaline, pale brown to brown hyphae.

Material examined: THAILAND, Chiang Rai, Muang, Doi Tung, elev. ca. 1,509 mls., on dead wood of PinusL. (Pinaceae),6 November 2009, Saranyaphat Boonmee DT-06 (MFLU10–0049, holotype= PDD 104451, isotype), ex-type cultures = MFLUCC10–0116 = BCC 52036= IFRD 2196.

 Fig. 1 Acanthohelicospora pinicola(MFLU10–0049, holotype). a Superficial ascomata on substrate. Note ascomata surrounded by black setae. b L.S. of ascoma. c Peridium with dark setae. Single seta. e Pseudoparaphyses. f,g Asci. h-m Ascospores. Scale bars: a-b=100µm, c-d, h-m=20µm, e=5µm, f-g=50µm


Fig. 2 Acanthohelicospora pinicola(MFLU10–0049, holotype). Colonies and asexual state on MEA culture. a Germinating ascospore. b, c Colonies on MEA from surface and reverse. Note dark brown colonies. d Arial mycelium on culture. e-g Conidiophores developing on hyphae. h Conidiogenous cells. i-l Conidia. Scale bars: a-c=10mm, d-i=5µm, e-l=10µm