Amniculicola Y. Zhang & K.D. Hyde, Mycol. Res. 112(10): 1189 (2008), MycoBank: MB 511328
Saprobic on wood in freshwater habitats. Sexual state: Ascomata scattered, solitary, or in small groups, initially immersed, becoming erumpent, to nearly superficial, with basal wall remaining immersed in host tissue, laterally flattened and base not easy to remove from the host, with black wall, rough and often consisting of remnants of the host, usually staining the woody substrate purple, globose to subglobose, broadly or narrowly conical, ostiolate. Ostiole slit-like, with apex well-differentiated into two tuberculate flared lips, long, ostiolar canal filled with periphyses. Peridium thick laterally and at the apex, thinner at the base, coriaceous, two-layered, outer layer composed of small heavily pigmented thick-walled cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis . Hamathecium comprising numerous, hyaline, septate, narrow, trabeculate, branched pseudoparaphyses, anastomosing between and above the asci, and embedded in gel matrix. Asci 8-spored, bitunicate, fissitunicate, long cylindrical to narrowly fusiform, with a short, twisted, narrow, furcate pedicel, truncate to rounded at the apex with a minute ocular chamber, inconspicuous when observed in water. Ascospores obliquely uni-seriate, partially overlapping, or bi-seriate, broadly fusiform to fusiform with broadly to narrowly rounded ends, hyaline, 1-septate, deeply constricted at the median septum, smooth-walled, containing four refractive oil globules, surrounded by an irregular, hyaline, gelatinous sheath, observed only when mounted in Indian ink. Asexual state: Unknown.
Notes : Based on ascomata with slit-like ostioles, thin, branching and anastomosing pseudoparaphyses and 1-septate, deeply constricted, fusiform ascospores, with a wide gelatinous sheath, Zhang et al. (2008b) introduced this new freshwater genus and referred it to the Pleosporales (Zhang et al. 2009a, 2012a). Initially treated as monotypic, the genus Amniculicola is represented by A. lignicola Y. Zhang ter & K.D. Hyde, but subsequently two other species were described, A. immersa Y. Zhang ter. et al. and A. parva Y. Zhang ter. et al. (Zhang et al. 2009b). All three Amniculicola species were collected from submerged wood in freshwater (Zhang et al. 2008b, 2009b). Shearer et al. (2009) showed that Amniculicola parva , Anguillospora longissima and Amniculicola immersa formed well-supported sister group to Amniculicola lignicola, with Massariosphaeria typhicola (P. Karst.) Leuchtm. (mistakenly designated as Semimassariosphaeria typhicola in Shearer et al. 2009) in a sister clade. In a four gene analysis Repetophragma ontariense, A. immersa, A. parva formed a sister group to Massariosphaeria (current name Neomassariosphaeria ) typhicola and M. grandispora (Sacc.) Leuchtm. in a wellsupported clade (Zhang et al. 2012a). However, the type species A. lignicola was not included in their analysis. The sexual state of Anguillospora longissima, has been reported as an undescribed species of ‘Massarina’ (Willoughby and Archer 1973; Webster 1992), which possesses superficial ascomata with a dark peridium, cylindrical, 8-spored asci and fusiform 1-septate ascospores, characters compatible with the morphological characters similar to Amniculicola lignicola . Therefore the sexual state of Anguillospora longissima may be Amniculicola parva, or a new species of Amniculicola, however this cannot currently be resolved as no asexual state has been reported for Amniculicola lignicola.
Type species : Amniculicola lignicola Y. Zhang ter & K.D. Hyde, Mycol. Res. 112(10): 1189 (2008), MycoBank MB511330

Fig. Amniculicola lignicola (Material examined: FRANCE, Rimont, Ruisseau de Peyrau on driftwood of Alnus glutinosa, 23 July 2006, J. Fournier,HKU(M) 17515, holotype). a, b Placement of the ascomata on host surface. c Close up of ascomata with slit like opening. d, e Section of ascoma (TS) with purple basal consisting of remnants of the host. f Close up of the peridium. g Slot-like ostiole, apex well-differentiated into two tuberculate flared lips. h Septate, long, hyaline, anastomosing and branched pseudoparaphyses placing between and above the asci. i -l Eight-spored asci with short, furcate pedicel. m-o Hyaline ascospores, with deeply constricted median septa. p Ascospores surrounded by an irregular hyaline gelatinous sheath in Indian ink. Scale bars: d–e = 150μm, f =25μm, g =40μm, h =5μm, i–l =50μm, m–p =10μm